To make a diagnosis about the carrier of the hepatitis C virus, it is necessary to undergo a number of examinations. First of all, it is necessary to pass a blood test for the so-called hepatic profile, including such biochemical studies as AST (aspartate aminotransferase), ALT (alanine aminotransferase), total and direct bilirubins, albumin, total protein, GGT (gammaglutamiltransferase), schf (alkaline phosphatase), and perform a General blood test with leukoformula. Another important method is ultrasound of the liver and abdominal organs. These studies will assess the functional and morphological state of the liver, to judge the ongoing inflammatory processes in it. However, it should be understood that it is impossible to diagnose the carrier of hepatitis C virus according to the studies listed above.
This task requires more precise methods of virus detection. The main screening study is an ELISA for the presence of antibodies specific to hepatitis C virus.depending on the manufacturer of the test system, IgG or IgM antibodies can be determined, but most often a combination of antibodies is used in commercial kits. If the result is positive, a confirmation test is performed on the ELISA. If the confirmatory test is also positive, the diagnosis is considered to be confirmed. In addition, it is possible to determine the virus by PCR (polymerase chain reaction) for the presence of its RNA. The PCR method is also very accurate, as it allows to qualitatively, if necessary, quantify the RNA content of the virus. Genotyping viral RNA is used to determine the genotype and subtypes required to determine the most appropriate course of treatment. If the result of PCR was positive, it is believed that the diagnosis was confirmed.